Transactions of the National Academy of Science and Technology
Catap, Elena S.
Canonizado, Excelle Grace M.
Many antioxidants that confer protection through inhibition of cellular membrane damage have been developed from various plants. In this study, the antioxidant properties of the leaf extract of Ficus septica, and the vine bark extract from Uncaria perrottetti. were assessed by measuring the malondialdehyde (MDA) levels, as by-product of lipid peroxidation, in the liver of ICR mice. In addition, histological examination was undertaken to determine the hepatoprotective effects of the two plant extracts. Mice were grouped into five cages, as follows: 1) negative control-phosphate buffered saline (PBS); 2) negative control-corn oil only 3) positive control-intraperitoneal injection of 30% carbon tetrachloride (CC14) in corn oil (1 ml/kg BW) every 72 hours; 4) treatment with U. perrottetti + CC14; and 5) treatment with F. septica + CC14. Both crude extracts were injected at a dosage of 250 mg/kg BW, and were given daily through intraperitoneal injections for two weeks. Results obtained from the lipid peroxidation assay showed that the CC14-treated mice had the highest MDA levels. Lower MDA levels were detected in the extract-treated mice and in the negative control mice but these were not statistically significant. However, histological examinations revealed various signs of cellular repair in the hepatic tissues of the extracted-treated mice relative to the degenerative and necrotic pathologies in the CC14-injected mice. The results show that the extracts conferred hepatoprotection but future validation studies must employ a longer period of treatment and other routes of extract application. (Author's abstract)