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TITLE       DNA mobility shift assay as a tool for the detection of anti-dsDNA antibodies in sera from discoid lupus erythematosus patients
CALL NO(S)      F(S) RL1 J272
LOCATION(S)      STII 
PUBLICATION TITLE      The Journal of Dermatology
VOLUME/ISSUE      39(7)
ISSUE DATE      Jl 2012
PAGINATION/COLLATION      602-607
MAIN AUTHOR      Keyhani, Jacqueline
ADDED ENTRY      Keyhani, Ezzatollah, Ahadi, Mashallah, Naraghi, Zahra, Shamohammadi, Safar
ABSTRACT      Discoid lupus erythematosus (DLE) is a form of local inflammatory autoimmune disease limited to the skin, involving essentially the face, scalp and ear.  DLE occurs in genetically predisposed individuals, sunlight being an identified trigger.  Diagnosis is made by clinical examination and histopathology; laboratory tests occasionally performed include anti-nuclear antibodies titers and presence of circulating antibodies against dsDNA.  DLE patients have about a 10% chance of developing systemic lupus erythematosus (SLE), a systemic inflammatory autoimmune disease affecting a range of only 20-55% of DLE patients by routine laboratory tests such as enzyme-linked immunosorbent assay (ELISA).  In this research, we applied an electrophoretic mobility shift assay (EMSA) in parallel with an ELISA for the detection of circulating anti-dsDNA in DLE patients. Results suggest that the EMSA is more sensitive than the routine tests used for the detection of anti-dsDNA in DLE, thus helping to improve early detection of the disease and, by extension, to better evaluate the factors triggering the disease.  (Author's abstract)
SUBJECTS       Medicine
   Circulating anti-dsdna antibodies
   Discoid lupus erythematosus
   Dna-immunoglobulin g complex
   Electrophoretic mobility shift assay
   Histopathology
   
 
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