The objective of the study is to fractionate and isolate secondary metabolites from Eleusine indica for the purpose of partially validating its reported diuretic property. Specifically it sought to test the diuretic activity in vivo of the crude extracts and the fractions thereof. To determine the type of diuretic present in the extract and to identify the diuretic component. The study also initiates a pool of secondary metabolites of the plant by obtaining relatively pure isolates for spectroscopic characterization.
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