The effects of Aglaia loheri Blanco, Aleurites moluccana Linn. and Ardisia pyramidalis (Cavs.) Pers. plant extracts on hepatic histology and lipid peroxidation activity in carbon tetrachloride-treated mice
Transactions of the National Academy of Science and Technology
Catap, Elena S.
Ragudo, Michelle Alisa DC.
Three plant species, Aglaia loheri, Aleurites moluccana, and Ardisia pyramidalis were evaluated for their antioxidant properties by employing lipid peroxidation assays and histological analysis in carbon tetrachloride (CC14)-treated mice. Sixty mice were divided into six treatment groups: 1) PBS-injected mice; 2) corn oil injected mice; 3) 30% CC14 in corn oil intraperitoneally injected in mice every 72 hrs; 4) A. loheri extract + CC14; 5) A. moluccana + CC14; 6) A. pyramidalis + CC14. Each of the plant extract was injected intraperitoneally on a daily basis. After two weeks, liver tissue were dissected out and subjected to lipid peroxidation assays. Liver samples were also processed for histopathological examination. Lipid peroxidation in both control groups was significantly lower than in CC14-treated mice. Malondialdehyde (MDA) levels in Ardisia-treated and Aleurites-treated mice are higher than the CC14-treated mice but the Aglaia-treated mice had lower MDA levels than the CC14-treated mice. However, the difference was not statistically significant. In the histological examination, the extracts from Ardisia and Aleurites seemed to induce cellular regeneration in CC14-treated mice. Higher degree of vacuolization and nuclear pyknosis, ballooning degeneration and multifocal necrosis were prominent in CC14-treated liver tissue. Based on these results, it is recommended that further studies to confirm the antioxidant capacity of these plant species should employ varying extract dosages and longer treatment periods. (Author's abstract)