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TITLE       Transovarial transmission of dengue virus in Aedes aegypti in Quezon City
CALL NO(S)      Fil(S) R97.4 U37 3/1 2014
LOCATION(S)      STII 
PUBLICATION TITLE      UERM Health Science Journal
VOLUME/ISSUE      3(1)
ISSUE DATE      2014 January to June
PAGINATION/COLLATION      1-8
MAIN AUTHOR      Bawalan, Ralph Julius
ADDED ENTRY      Salazar, Nelia P.
ABSTRACT           Introduction: Due to the changing nature of dengue epidemiology and control, this study was conducted to det ermine and describe evidence for transovarial transmission of the dengue virus.

     Methods: This was a quantitative experimental study on the transovarial transmission of the dengue virus from field-collected Aedes aegypti in an animal model. Viremia was detected by reverse transcriptase-polymerase chain reaction. Mosquito homogenate was used for intracranial inoculation of the virus into suckling mice. The brains of the suckling mice were extracted and inoculated intraperitoneally into 3 to 4 week old mice for recording of disease manifestations.

     Results: The mice infected intraperitoneally with dengue virus from field Aedes mosquitoes showed evidence of dengue disease manifested through physical signs, thrombocytopenia and histopathologic changes in affected organs.

     Conclusion: These observations indicate that transovarial transmission of dengue virus can occur in a highly urbanized locale like Quezon City where dengue cases are high , and virulence may translate into dengue disease when inoculated in an animal model. (Authors' abstract)
SUBJECTS       Medicine
   Dengue
   Animal model
   Vector control
   Histopathology
   Molecular diagnostic
   Virus inoculation
   
 
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