Introduction: Due to the changing nature of dengue epidemiology and control, this study was conducted to det ermine and describe evidence for transovarial transmission of the dengue virus.
Methods: This was a quantitative experimental study on the transovarial transmission of the dengue virus from field-collected Aedes aegypti in an animal model. Viremia was detected by reverse transcriptase-polymerase chain reaction. Mosquito homogenate was used for intracranial inoculation of the virus into suckling mice. The brains of the suckling mice were extracted and inoculated intraperitoneally into 3 to 4 week old mice for recording of disease manifestations.
Results: The mice infected intraperitoneally with dengue virus from field Aedes mosquitoes showed evidence of dengue disease manifested through physical signs, thrombocytopenia and histopathologic changes in affected organs.
Conclusion: These observations indicate that transovarial transmission of dengue virus can occur in a highly urbanized locale like Quezon City where dengue cases are high , and virulence may translate into dengue disease when inoculated in an animal model. (Authors' abstract)